| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140522 |
| Chromosome: | chromosome 16 |
| Location: | 705237 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691350 | (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTTGCAGGAGCAGAGCAACGCCTGCAGT |
| Internal bar code: | CCCGGACGCGGCATGCTTAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1079 |
| LEAP-Seq percent confirming: | 99.534 |
| LEAP-Seq n confirming: | 7049 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGGCTCATCAACAAGTA |
| Suggested primer 2: | GATGCACCGTGTAGGTGTTG |