| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.140545 |
| Chromosome: | chromosome 10 |
| Location: | 1102678 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425501 | (1 of 1) K03125 - transcription initiation factor TFIID subunit 1 (TAF1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACACCACACACCGAGTTGAGCCCGCCGT |
| Internal bar code: | GATCGGGAGTAGGGGGGAATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 140 |
| LEAP-Seq percent confirming: | 93.6634 |
| LEAP-Seq n confirming: | 473 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCCAACTTCGCTTAGTC |
| Suggested primer 2: | ATATGACCAAGCCCAACGAG |