| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.140548 |
| Chromosome: | chromosome 16 |
| Location: | 4536915 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g667150 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGTTGGGGGACAAAGAGTCGCGGCCCT |
| Internal bar code: | GGAGGGCACCGTTAGAGGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 735 |
| LEAP-Seq percent confirming: | 75.9002 |
| LEAP-Seq n confirming: | 2129 |
| LEAP-Seq n nonconfirming: | 676 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTTCCGAAGCCAAACAC |
| Suggested primer 2: | ACAGATGCTCTAGACGCGGT |