| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.140587 |
| Chromosome: | chromosome 16 |
| Location: | 1316271 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651650 | TSI3 | Isoleucyl-tRNA synthetase; (1 of 3) 6.1.1.5 - Isoleucine--tRNA ligase / Isoleucyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCGGGCACAGCGCTGGCGGGCGAGCGGC |
| Internal bar code: | CATGTGCTGAAATAGGACTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 552 |
| LEAP-Seq percent confirming: | 99.4156 |
| LEAP-Seq n confirming: | 1361 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGTTTTGGAGGTTGCAC |
| Suggested primer 2: | TGGCTTGAATATCCCTTTGG |