Insertion junction: LMJ.RY0402.140624_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.140624
Chromosome:chromosome 6
Location:3925346
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278186 (1 of 1) IPR000157//IPR011251//IPR016024 - Toll/interleukin-1 receptor homology (TIR) domain // Luciferase-like domain // Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACTTGATAGCTAGATCTTCACATCTCGTG
Internal bar code:TTGTACTATGGAGAGCGCACCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1057
LEAP-Seq percent confirming:86.5385
LEAP-Seq n confirming:90
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGTATTGCTAGGCCTTCG
Suggested primer 2:GAGCGAGCAGAGAGCAGAAT