Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.140624 |
Chromosome: | chromosome_6 |
Location: | 3925346 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g278186 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACTTGATAGCTAGATCTTCACATCTCGTG |
Internal bar code: | TTGTACTATGGAGAGCGCACCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1057 |
LEAP-Seq percent confirming: | 86.5385 |
LEAP-Seq n confirming: | 90 |
LEAP-Seq n nonconfirming: | 14 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGAGTATTGCTAGGCCTTCG |
Suggested primer 2: | GAGCGAGCAGAGAGCAGAAT |