| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140636 |
| Chromosome: | chromosome 16 |
| Location: | 3666642 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688600 | XPG4 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGATTTATTCGGCAGCGGTTCAACAGC |
| Internal bar code: | CAAGTTGTCTCTTATGAGCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 159 |
| LEAP-Seq percent confirming: | 99.5789 |
| LEAP-Seq n confirming: | 946 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCACTCCATACCTGCCTGG |
| Suggested primer 2: | ATAAAAGAATCCCCCGCATC |