| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.140675 |
| Chromosome: | chromosome 10 |
| Location: | 5482369 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458950 | IBA57 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGATCATTCCACCCTCCAAGCACAACCCTC |
| Internal bar code: | TCTCAGCTCGAGGGACGAAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 93.1751 |
| LEAP-Seq n confirming: | 2198 |
| LEAP-Seq n nonconfirming: | 161 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGTCACTTGTGCGGGAT |
| Suggested primer 2: | ACATTGGTACAGCAGGGGAG |