| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.140703 |
| Chromosome: | chromosome 12 |
| Location: | 5569724 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531651 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCTGCTCCGCGTCCTCTAAAAGGCGTG |
| Internal bar code: | ACAGCGCGCGGAGTCTGGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 737 |
| LEAP-Seq percent confirming: | 98.6755 |
| LEAP-Seq n confirming: | 894 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTTATCCTCTGCTTCAC |
| Suggested primer 2: | ATCCAAATGCTGCTCCAAAC |