| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.140715 |
| Chromosome: | chromosome 6 |
| Location: | 8652284 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309450 | CPZ | (1 of 1) 3.4.17.12 - Carboxypeptidase M | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCATCGCCATCTTCCACACCAGGCCGC |
| Internal bar code: | CTCAGCGTCCGTAGCGAGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 209 |
| LEAP-Seq percent confirming: | 99.2968 |
| LEAP-Seq n confirming: | 4660 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGAGCCTAACCGTCAAA |
| Suggested primer 2: | GCGCAAGTAGGCTGGTAGAC |