| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140730 |
| Chromosome: | chromosome 2 |
| Location: | 4473030 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g100300 | (1 of 1) PTHR15245:SF11 - PHOSPHATIDYLINOSITOL 3-KINASE-RELATED PROTEIN KINASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTCCAGGCGGTGCAGAGTTCTGCTGGT |
| Internal bar code: | TCGTTTAAGGTCCAAGTTTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 99.6063 |
| LEAP-Seq n confirming: | 253 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATTGCTGGGACTGGTTGT |
| Suggested primer 2: | ATACAGACGTGGACATGGCA |