| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140752 |
| Chromosome: | chromosome 2 |
| Location: | 336119 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g075550 | (1 of 1) PTHR28532//PTHR28532:SF1 - FAMILY NOT NAMED // ORAL CANCER-OVEREXPRESSED PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGATATGAAGAACGTCTGCTTGCCTTGGA |
| Internal bar code: | GTCTCAGGCTTCGCCTTCCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 893 |
| LEAP-Seq percent confirming: | 99.8544 |
| LEAP-Seq n confirming: | 686 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTACGGCGACTTTGAAC |
| Suggested primer 2: | TTACACGGGGTTGAAAGGAG |