| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.140796 |
| Chromosome: | chromosome 13 |
| Location: | 3769916 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589650 | (1 of 1) PF08424 - NRDE-2, necessary for RNA interference (NRDE-2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGGTAGGCAACCCACACCGGGGCCACGT |
| Internal bar code: | AGGCCTATCGGTACACCGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 270 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 260 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCCGGACTCCTTTCACTG |
| Suggested primer 2: | TCCATTCCCATCTTCTCCTG |