| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.140812 |
| Chromosome: | chromosome 2 |
| Location: | 7409542 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144600 | PTB9 | Sodium/phosphate symporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGGCTGGGTAATTGTTTTAGTGCATTT |
| Internal bar code: | AGGGTCAAGAGAGTGGATGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 58 |
| LEAP-Seq percent confirming: | 85.443 |
| LEAP-Seq n confirming: | 135 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATCGGCGCTAATGATGGT |
| Suggested primer 2: | ACCAACCCAACTCACTCGTC |