| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.140836 |
| Chromosome: | chromosome 8 |
| Location: | 3899179 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378150 | GLD2 | Glucose-6-phosphate dehydrogenase; (1 of 2) 1.1.1.49 - Glucose-6-phosphate dehydrogenase (NADP(+)) / GPD | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGCCTTACAGTGCGGCGCCTCTCAACC |
| Internal bar code: | CAAGACCCAGGATAATCTCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 907 |
| LEAP-Seq percent confirming: | 93.4898 |
| LEAP-Seq n confirming: | 2987 |
| LEAP-Seq n nonconfirming: | 208 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTAGGCAAGTACGAGGG |
| Suggested primer 2: | TTGACGTCTGCCTATGCTTG |