Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.140844 |
Chromosome: | chromosome_3 |
Location: | 6483434 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g194900 | CYA4 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTCAAGCTGCAACCTCGTTGTTGAGCGTGT |
Internal bar code: | GTGTGTGTAGGGTTCACCGTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 429 |
LEAP-Seq percent confirming: | 98.6023 |
LEAP-Seq n confirming: | 1552 |
LEAP-Seq n nonconfirming: | 22 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTAATCTCCTGCAAGACGC |
Suggested primer 2: | GCATAAGGCATAGCTGGCTC |