| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.140851 |
| Chromosome: | chromosome 2 |
| Location: | 6539262 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g116850 | HLM6,SETb | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase; Histone-lysine N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTACCGCGCCCTCTCCACGTCTGCAGC |
| Internal bar code: | CGGCTTATGAATCTAGTTAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 243 |
| LEAP-Seq percent confirming: | 34.4828 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGGTACAATTTGGGCAG |
| Suggested primer 2: | TATTGTTGTCGAGCAGGCAG |