| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140857 |
| Chromosome: | chromosome_9 |
| Location: | 656244 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g403200 | FAP198 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGGGGGGGTTGTCGGATGGGCGACAAAGG |
| Internal bar code: | ACAAGTTTCTATACAGGACTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 99.7152 |
| LEAP-Seq n confirming: | 2801 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCACTTCCAGCAGCTTCC |
| Suggested primer 2: | GTCTCCTTCCCTTGCTCCTT |