| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.140882 |
| Chromosome: | chromosome 7 |
| Location: | 1929263 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325748 | (1 of 1) 3.5.2.9 - 5-oxoprolinase (ATP-hydrolyzing) / Pyroglutamase (ATP-hydrolyzing) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGCCGTGGCACACCACTTCCACCGACG |
| Internal bar code: | GAACTTAGAGTCCGCCCGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 575 |
| LEAP-Seq percent confirming: | 72.4873 |
| LEAP-Seq n confirming: | 714 |
| LEAP-Seq n nonconfirming: | 271 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGAAAATAGACAGCACA |
| Suggested primer 2: | AATCAACCGAGTCCTTGTCG |