| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.140900 |
| Chromosome: | chromosome 3 |
| Location: | 2660620 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g161000 | ADA1 | Adenosine/AMP deaminase; (1 of 1) 3.5.4.4 - Adenosine deaminase / Adenosine aminohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTGGTCCCCCACACCCCGTGAGTCCCC |
| Internal bar code: | TTCGATTTCTAGGGGTGCTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 530 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 201 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGACATGGTGTGCAAATG |
| Suggested primer 2: | TATAGCCTGGAGGGTGTGCT |