| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.140905 |
| Chromosome: | chromosome 4 |
| Location: | 2820264 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224150 | (1 of 1) 2.7.1.30 - Glycerol kinase / Glycerokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGCTACACTTCTCTGTACCAATCTTCG |
| Internal bar code: | GAACCGTTGTGTGCGCCTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 89.3789 |
| LEAP-Seq n confirming: | 17773 |
| LEAP-Seq n nonconfirming: | 2112 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGTAAGAAGGAGGAGGG |
| Suggested primer 2: | TCCACTGCAACCCTAAAACC |