Insertion junction: LMJ.RY0402.140913_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.140913
Chromosome:chromosome 7
Location:1921048
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g325744 (1 of 1) PTHR19277//PTHR19277:SF102 - PENTRAXIN // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTACCAAACCCAAACGGCGACACCAGGCC
Internal bar code:TAGCGTATAAGACGGATTTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:157
LEAP-Seq percent confirming:97.7233
LEAP-Seq n confirming:558
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCGACCCTATGTCCTTGT
Suggested primer 2:TGTACTTCCGCTGCAAACAG