| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.140914 |
| Chromosome: | chromosome 3 |
| Location: | 329598 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144687 | (1 of 2) PTHR12983:SF9 - RING FINGER PROTEIN 10 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGTTGGGCAGTGGTTTGAAGAGGGGAAG |
| Internal bar code: | AGTGGGTTAGCGGAAGATCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 841 |
| LEAP-Seq percent confirming: | 93.3958 |
| LEAP-Seq n confirming: | 13859 |
| LEAP-Seq n nonconfirming: | 980 |
| LEAP-Seq n unique pos: | 108 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGACCCTGTTGATTGGG |
| Suggested primer 2: | ATGTGATTTTCGTCATGGCA |