| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.140927 |
| Chromosome: | chromosome 8 |
| Location: | 3544277 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375450 | SDR13 | (1 of 1) 1.1.1.243 - Carveol dehydrogenase; Short-chain dehydrogenase/reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTTGGCTCACTGCAGAACAGGCTGATGT |
| Internal bar code: | GCAGACACTCGACAAGGCAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 767 |
| LEAP-Seq percent confirming: | 91.5496 |
| LEAP-Seq n confirming: | 3911 |
| LEAP-Seq n nonconfirming: | 361 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCCTTCTCAGCCTCCAG |
| Suggested primer 2: | CAAACAAGCGCAGAGTGTGT |