Insertion junction: LMJ.RY0402.140927_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.140927
Chromosome:chromosome 9
Location:6564048
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g408051 (1 of 2) K15032 - mTERF domain-containing protein, mitochondrial (MTERFD) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCACCCGCCAGCGGCAGTAGCTGCCGCT
Internal bar code:GACGTCCGTATGACCGGCTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:346
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:486
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCATCATCACCACCTCCT
Suggested primer 2:TACCTCACCACAGTACGGCA