Insertion junction: LMJ.RY0402.140958_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.140958
Chromosome:chromosome 5
Location:805056
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g248200 (1 of 2) PTHR21493//PTHR21493:SF124 - CGI-141-RELATED/LIPASE CONTAINING PROTEIN // SUBFAMILY NOT NAMED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTTGAGGTGACGTCGGCAGGCTTTAATG
Internal bar code:GACGCTTTGAGGGGGGCTGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:855
LEAP-Seq percent confirming:72.8898
LEAP-Seq n confirming:734
LEAP-Seq n nonconfirming:273
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CAACAGTGGGGCACCTATCT
Suggested primer 2:GTCAGCGCTATGATGCATGT