Insertion junction: LMJ.RY0402.140965_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.140965
Chromosome:chromosome 2
Location:6375999
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g115400 (1 of 2) IPR002889//IPR008979 - Carbohydrate-binding WSC // Galactose-binding domain-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTGACCCCACGCTGCCACACTGACCAAG
Internal bar code:CACGGCTGCTCAGCTGTCGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:841
LEAP-Seq percent confirming:99.4792
LEAP-Seq n confirming:764
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTAGCACTGTGGCGTTGA
Suggested primer 2:AATCGGTTCCTGTTTGCTTG