| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.140982 |
| Chromosome: | chromosome 7 |
| Location: | 1232446 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321350 | (1 of 2) 3.1.3.1 - Alkaline phosphatase / Phosphomonoesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCTGTGTTAGCCCCCATTGTTCGAATC |
| Internal bar code: | GCGCTGACTGAAATAAGGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 31 |
| LEAP-Seq percent confirming: | 86.8217 |
| LEAP-Seq n confirming: | 1456 |
| LEAP-Seq n nonconfirming: | 221 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGTTCTGGTCGCCACAT |
| Suggested primer 2: | CCAGACTAAACCGAACCCAA |