| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.140987 |
| Chromosome: | chromosome 5 |
| Location: | 1148293 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245701 | FAP104 | Flagellar Associated Protein 104 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTCAAGGGGTAGGAGCGGAGAGGGCCT |
| Internal bar code: | GCCGGAATAACGTGGGGCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 678 |
| LEAP-Seq percent confirming: | 99.6063 |
| LEAP-Seq n confirming: | 2024 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTTTCAACTCTGAGCCC |
| Suggested primer 2: | AGCCCAACTACGACCACATC |