| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.140992 |
| Chromosome: | chromosome 12 |
| Location: | 3932406 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516250 | (1 of 25) PTHR10516 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAAGTGGGGGCATGCACAGATGAATGCCC |
| Internal bar code: | GTCCGGGCATGGCAAAGCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 99.7145 |
| LEAP-Seq n confirming: | 8382 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGCGAAGAGTAACGAGA |
| Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |