| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141000 |
| Chromosome: | chromosome 11 |
| Location: | 3615944 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482050 | FAL13 | Protein of unknown function; (1 of 1) PF09751 - Nuclear protein Es2 (Es2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGGCCTGTCCATGACACAACTGCCATG |
| Internal bar code: | AAGCCTACGTAACGAAGGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 225 |
| LEAP-Seq percent confirming: | 97.585 |
| LEAP-Seq n confirming: | 1980 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGTTGATGGACTTGGGC |
| Suggested primer 2: | CACTCCGAAACTGGCTTTTC |