| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.141052 |
| Chromosome: | chromosome 12 |
| Location: | 4217525 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518800 | (1 of 2) PTHR11132:SF126 - PROTEIN C29A12.6, ISOFORM B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACTGCGCGACTCGACAGGTCCGACAGCA |
| Internal bar code: | GGTTCTGCTGGTCCAAGGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 820 |
| LEAP-Seq percent confirming: | 99.7266 |
| LEAP-Seq n confirming: | 2553 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTGTAGCCAGCTTCTCC |
| Suggested primer 2: | GCCTCAAGCACTGAATAGCC |