| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.141087 |
| Chromosome: | chromosome_6 |
| Location: | 4067580 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g278212 | CGL46 | Predicted protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTCGAACAGGTCCTTGCCCATGCAGTCCC |
| Internal bar code: | GCTCTAGTCGTATGTCCTGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 868 |
| LEAP-Seq percent confirming: | 99.3528 |
| LEAP-Seq n confirming: | 614 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGGTGCTGATGGTGATG |
| Suggested primer 2: | GCGGAAAGCTACAGTTCCTG |