| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.141090 |
| Chromosome: | chromosome 12 |
| Location: | 3914446 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516000 | (1 of 2) PF07978 - NIPSNAP (NIPSNAP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTTCACCGACGACCGAGCGGGACGCAG |
| Internal bar code: | GGAGGCATTTTTGTAAACCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 849 |
| LEAP-Seq percent confirming: | 99.6052 |
| LEAP-Seq n confirming: | 1766 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCAGGCTTCGGAGCTTAT |
| Suggested primer 2: | TTTCGCTACTGAATTGCACG |