Insertion junction: LMJ.RY0402.141094_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.141094
Chromosome:chromosome 16
Location:5956858
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g676850 (1 of 14) IPR003593//IPR003959//IPR027417 - AAA+ ATPase domain // ATPase, AAA-type, core // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACCTCGCGCGCCATGGCCCGCAGCTCGG
Internal bar code:CGATCACTCTCAAACAGGCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:425
LEAP-Seq percent confirming:99.1209
LEAP-Seq n confirming:902
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CAATACACACACGCTCCCAC
Suggested primer 2:CAACGATGTCTGTGGAGTGG