| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.141117 |
| Chromosome: | chromosome 13 |
| Location: | 1452478 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572200 | (1 of 1) K03834 - tyrosine-specific transport protein (tyrP) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTTGGTTTATTGTCAGCGCATTACTGA |
| Internal bar code: | GAAGCGTGGCGAGTACGGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 903 |
| LEAP-Seq percent confirming: | 99.9063 |
| LEAP-Seq n confirming: | 4267 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAACGTAGGCTGCTCCTT |
| Suggested primer 2: | CTAGTCAGCTGCACCATCCA |