| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141131 |
| Chromosome: | chromosome 16 |
| Location: | 1919720 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656050 | (1 of 26) IPR000209 - Peptidase S8/S53 domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATGGTGCACGCCGCGTGTGTTGACAGA |
| Internal bar code: | AGTAGTGATGGTGGGCTTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1028 |
| LEAP-Seq percent confirming: | 80.8383 |
| LEAP-Seq n confirming: | 2430 |
| LEAP-Seq n nonconfirming: | 576 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGTTGTTCTCCCCTTTC |
| Suggested primer 2: | ATTTTCCCAAAAATCCCGAC |