| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141141 |
| Chromosome: | chromosome 3 |
| Location: | 4031828 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172250 | (1 of 1) PTHR11649:SF5 - GTP-BINDING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCGCCTGCCGGCCTGTTGGCTTTGCTA |
| Internal bar code: | TCCCCAGATATGGGGATGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 91.227 |
| LEAP-Seq n confirming: | 3026 |
| LEAP-Seq n nonconfirming: | 291 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGGATGCTGTTGTGCAT |
| Suggested primer 2: | ACATTCGCCGCTAGATGAGT |