Insertion junction: LMJ.RY0402.141144_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.141144
Chromosome:chromosome 1
Location:3982632
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g025900 (1 of 7) PF00415//PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1) // Regulator of chromosome condensation (RCC1) repeat (RCC1_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTATTTATAACCCCACCCCAGGGCCTCC
Internal bar code:AGAGGTCGAGCGATGCGGTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:147
LEAP-Seq percent confirming:99.569
LEAP-Seq n confirming:231
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TACCAAAGGACTGGACCTCG
Suggested primer 2:TTTCGTCTTTTATGGACGGC