| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.141156 |
| Chromosome: | chromosome 7 |
| Location: | 5226950 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348900 | DNJ19 | (1 of 1) K09537 - DnaJ homolog subfamily C member 17 (DNAJC17); DnaJ-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGTATCCCCTCGTGGTTAGTGGTTGCGG |
| Internal bar code: | GGTGATACACGCCGGGACTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1108 |
| LEAP-Seq percent confirming: | 99.7312 |
| LEAP-Seq n confirming: | 14840 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAGAGGAGAGGAGAGCAT |
| Suggested primer 2: | ATAACAACCAACGGCTTTCG |