| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141194 |
| Chromosome: | chromosome 16 |
| Location: | 1929717 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656200 | (1 of 1) PF00612//PF13424 - IQ calmodulin-binding motif (IQ) // Tetratricopeptide repeat (TPR_12) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCCTAAATGCCGGGGCCAGCCGTTTGT |
| Internal bar code: | TGGTGGTAAGGACCTCGTAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 99.5546 |
| LEAP-Seq n confirming: | 447 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGGGTAGGGAGGGACTG |
| Suggested primer 2: | GGTGTCATGCTTGAACGCTA |