| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141209 |
| Chromosome: | chromosome 5 |
| Location: | 3391521 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241050 | (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGAGGGAGCGGGTTCGCGGTGCGTGAG |
| Internal bar code: | GGTTGAACCCCGTGTGTGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 91.4424 |
| LEAP-Seq n confirming: | 3772 |
| LEAP-Seq n nonconfirming: | 353 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAATCGTGTTGCGAGACAG |
| Suggested primer 2: | ATAAGCCAGTGCTTACGGGA |