| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.141209 |
| Chromosome: | chromosome 5 |
| Location: | 3391522 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241050 | (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCCTGAGGGCGACCGTGTTTGGTAGCC |
| Internal bar code: | GAGTCCAATTTACCTCGGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 860 |
| LEAP-Seq percent confirming: | 99.7689 |
| LEAP-Seq n confirming: | 3453 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAATCGTGTTGCGAGACAG |
| Suggested primer 2: | ATAAGCCAGTGCTTACGGGA |