Insertion junction: LMJ.RY0402.141213_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.141213
Chromosome:chromosome 3
Location:2183693
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g157450 (1 of 1) IPR015233 - Orange carotenoid-binding protein, N-terminal intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTGATGCAGACGCAAGACCGAACATAGG
Internal bar code:GAGTGGGGCGCGTCTCGGCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:723
LEAP-Seq percent confirming:99.7845
LEAP-Seq n confirming:926
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ACATGATGGCAGCACACAAT
Suggested primer 2:TGGCGGATATCACATCTCAA