Insertion junction: LMJ.RY0402.141233_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.141233
Chromosome:chromosome 9
Location:4832242
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g397512 HSPBP1 hsp70-binding protein 1; (1 of 1) K09562 - hsp70-interacting protein (HSPBP1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTCGTTGTTTTGCCCTCCGTCATCTCGG
Internal bar code:CCAGTCACACAAGGAGCCTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:201
LEAP-Seq percent confirming:52.3997
LEAP-Seq n confirming:1867
LEAP-Seq n nonconfirming:1696
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TACTCTGTCTCCCACCCGTC
Suggested primer 2:TGCTTGCGTGATAGAAATGC