| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.141249 |
| Chromosome: | chromosome 8 |
| Location: | 2152550 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g369250 | FAP400 | Flagellar Associated Protein 400 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCGTTGGAGCCAGCCTGGCCATGCTCGA |
| Internal bar code: | CGCCGGTTCCCAGGGGATGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 98.7557 |
| LEAP-Seq n confirming: | 3254 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATGGACTGCAGCGTGTA |
| Suggested primer 2: | CGCCTAGGTCACTCGCTTAC |