| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141260 |
| Chromosome: | chromosome 12 |
| Location: | 5576037 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531700 | ADA,ADA3 | Adenosine/AMP deaminase; (1 of 1) 3.5.4.6 - AMP deaminase / Myoadenylate deaminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCACTACTTCCGCCCCCGCTCACATCCA |
| Internal bar code: | CGAATCATGGGAGAGCAATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 109 |
| LEAP-Seq percent confirming: | 67.9245 |
| LEAP-Seq n confirming: | 144 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGCTCGCTCAAAATACAA |
| Suggested primer 2: | TGCTCATCTTGAACACCTGC |