| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141311 |
| Chromosome: | chromosome_10 |
| Location: | 5768271 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g461200 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCGGACAACGGTCGGCACGGCCGTTGCCG |
| Internal bar code: | GTATGGAAACCCTCCATTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 912 |
| LEAP-Seq percent confirming: | 97.1144 |
| LEAP-Seq n confirming: | 1851 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGAGCACCACAGAAGCAA |
| Suggested primer 2: | GGCTTAGATATGCTGGCTGC |