| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.141329 |
| Chromosome: | chromosome 10 |
| Location: | 3939318 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448350 | (1 of 3) PTHR10361 - SODIUM-BILE ACID COTRANSPORTER | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCACAGTAAGGCGGAGGATGAGCAGGA |
| Internal bar code: | GGGATACGGTCAGCTACCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 699 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGCACTTGCAGCATACG |
| Suggested primer 2: | CAAGCTGAGAACTGTCCGTG |