| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141331 |
| Chromosome: | chromosome 13 |
| Location: | 1925986 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576150 | APN1 | (1 of 1) PTHR11707//PTHR11707:SF3 - L-ASPARAGINASE // SUBFAMILY NOT NAMED; L-asparaginase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTCTGGTCCGGCAGGTTGCCCACCCCGA |
| Internal bar code: | GAGCGGTCGCAACTGGTGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 99.8296 |
| LEAP-Seq n confirming: | 586 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAACTACCGTAAGCCAA |
| Suggested primer 2: | ATGGTGGTGGTGGTGGTAGT |