| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.141407 |
| Chromosome: | chromosome_1 |
| Location: | 5497641 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g038750 | MOT7 | Predicted protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCACGGGGCAGACGTGCGGTTGGCGGAGAC |
| Internal bar code: | TCCGCCCGATGTTATCGTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 99.7961 |
| LEAP-Seq n confirming: | 4895 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCGAGTTCCCGTAAGCAG |
| Suggested primer 2: | CAGCTGCTAAAGGGCCTATG |